Tag Archives: Microscopy

Single frame wide-field Nanoscopy based on Ghost Imaging via Sparsity Constraints (GISC Nanoscopy)

This just got posted on the arXiv, and has some interesting ideas inside. Using a ground glass diffuser before a pixelated detector, and after a calibrating procedure where you measure the associated speckle patterns when scanning the sample plane, a single shot of the fluorescence signal speckle pattern can be used to retrieve high spatial resolution images of a sample.

Read more

Simultaneous multiplane imaging with reverberation multiphoton microscopy

Really nice pre-print by the people at Boston University, leaded by J. Mertz. Love the idea of generating ~infinite focal spots (until you run out of photons) inside a sample, and using a extremely fast single-pixel detector to recover the signal. Very original way to tackle volumetric imaging in bio-imaging! Simultaneous multiplane imaging with reverberation multiphoton microscopy by Devin R.

Read more

De-scattering with Excitation Patterning (DEEP) Enables Rapid Wide-field Imaging Through Scattering Media

Very interesting stuff from the people at MIT regarding imaging through scattering media. Recently, multiple approaches taking advantage of temporal focusing (TF) increased efficiency inside scattering media when using two-photon microscopy have been published, and this goes a step further. Here, the authors use wide-field structured illumination, in combination with TF, to obtain images with a large field-of-view and a

Read more

Wavefront correction in two-photon microscopy with a multi-actuator adaptive lens

The group leaded by P. Artal at Murcia University has recently published an interesting paper related to adaptive optics using an adaptive lens. When working in a real scenario, imperfections in the optical elements you use or just the objects you want to image introduce optical aberrations in the pictures you obtain. Usually these aberrations reduce the quality of your images

Read more

Weekly recap (29/04/2018)

This week we have a lot of interesting stuff: Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms Adaptive Optics + Light Sheet Microscopy to see living cells inside the body of a Zebra fish (the favorite fish of biologists!). Really impressive images overcoming scattering caused by tissue. You can read more about the paper on

Read more

Deep learning microscopy

This week a new paper by the group leaded by A. Ozcan appeared in Optica. Deep learning microscopy, Y. Ribenson et al, at Optica (featured image exctracted from Fig. 6 of the supplement) Abstract, We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field of view and depth of field. After

Read more